Review



human breast cancer cell lines mda mb 231  (ATCC)


Bioz Verified Symbol ATCC is a verified supplier
Bioz Manufacturer Symbol ATCC manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
    • 99
      Buy from Supplier

    Structured Review

    ATCC human breast cancer cell lines mda mb 231
    Ptch1 is expressed in breast cancer cell lines. A. PTCH1 mRNA expression (log 2 transformed) in various luminal and HER2 breast cancer cell lines (dark green) and in TNBC cell lines (other colors). TNBC cell lines are depicted according to the “Lehmann TNBC subtype” nomenclature : basal-like 1 (yellow), basal-like 2 (pale green), immunomodulatory (brown), luminal androgen receptor (dark pink), mesenchymal (pale pink) and mesenchymal stem-like (pink). B. PTCH1 protein expression in three TNBC cell lines. Western-blot was performed on 50 µg extracts from TNBC cell lines <t>(MDA-MB-231,</t> HCC-38 and MDA-MB-468) with antibodies directed against PTCH1. PTCH1 and GAPDH signals were quantified using ImageJ software. Data presented are the mean ± SEM of at least 3 independent experiments. Significance is calculated using Oneway ANOVA Turkey’s multiple comparisons test and attained at P < 0.05 (*).
    Human Breast Cancer Cell Lines Mda Mb 231, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 5685 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+breast+cancer+cell+line+mda+mb+231/MDA-MB-468/pmc13101699-47-0-12
    Average 99 stars, based on 5685 article reviews
    human breast cancer cell lines mda mb 231 - by Bioz Stars, 2026-09
    99/100 stars

    Images

    1) Product Images from "Inhibition of PTCH1 drug efflux activity enhances chemotherapy efficacy against triple negative breast cancers"

    Article Title: Inhibition of PTCH1 drug efflux activity enhances chemotherapy efficacy against triple negative breast cancers

    Journal: Translational Oncology

    doi: 10.1016/j.tranon.2026.102777

    Ptch1 is expressed in breast cancer cell lines. A. PTCH1 mRNA expression (log 2 transformed) in various luminal and HER2 breast cancer cell lines (dark green) and in TNBC cell lines (other colors). TNBC cell lines are depicted according to the “Lehmann TNBC subtype” nomenclature : basal-like 1 (yellow), basal-like 2 (pale green), immunomodulatory (brown), luminal androgen receptor (dark pink), mesenchymal (pale pink) and mesenchymal stem-like (pink). B. PTCH1 protein expression in three TNBC cell lines. Western-blot was performed on 50 µg extracts from TNBC cell lines (MDA-MB-231, HCC-38 and MDA-MB-468) with antibodies directed against PTCH1. PTCH1 and GAPDH signals were quantified using ImageJ software. Data presented are the mean ± SEM of at least 3 independent experiments. Significance is calculated using Oneway ANOVA Turkey’s multiple comparisons test and attained at P < 0.05 (*).
    Figure Legend Snippet: Ptch1 is expressed in breast cancer cell lines. A. PTCH1 mRNA expression (log 2 transformed) in various luminal and HER2 breast cancer cell lines (dark green) and in TNBC cell lines (other colors). TNBC cell lines are depicted according to the “Lehmann TNBC subtype” nomenclature : basal-like 1 (yellow), basal-like 2 (pale green), immunomodulatory (brown), luminal androgen receptor (dark pink), mesenchymal (pale pink) and mesenchymal stem-like (pink). B. PTCH1 protein expression in three TNBC cell lines. Western-blot was performed on 50 µg extracts from TNBC cell lines (MDA-MB-231, HCC-38 and MDA-MB-468) with antibodies directed against PTCH1. PTCH1 and GAPDH signals were quantified using ImageJ software. Data presented are the mean ± SEM of at least 3 independent experiments. Significance is calculated using Oneway ANOVA Turkey’s multiple comparisons test and attained at P < 0.05 (*).

    Techniques Used: Expressing, Transformation Assay, Western Blot, Software

    PTCH1 drug efflux inhibitor PAH increases the sensitivity of TNBC cells to chemotherapy . Cell viability was measured after 24 h or 48 h treatment with increasing concentration of docetaxel or doxorubicin respectively on MDA-MB-231, MDA-MB-468 and HCC-38 cell lines in the absence or the presence of 15µM PAH. IC 50 values (corresponding to the concentration of chemotherapy inducing 50% of cell death) were calculated. Data reported are the mean ± SEM of at least 3 independent experiments. Significance is attained at P < 0.05 (*).
    Figure Legend Snippet: PTCH1 drug efflux inhibitor PAH increases the sensitivity of TNBC cells to chemotherapy . Cell viability was measured after 24 h or 48 h treatment with increasing concentration of docetaxel or doxorubicin respectively on MDA-MB-231, MDA-MB-468 and HCC-38 cell lines in the absence or the presence of 15µM PAH. IC 50 values (corresponding to the concentration of chemotherapy inducing 50% of cell death) were calculated. Data reported are the mean ± SEM of at least 3 independent experiments. Significance is attained at P < 0.05 (*).

    Techniques Used: Concentration Assay

    Other multidrug transporters are expressed in TNBC cell lines. A. P-gp and ABCG2 protein expression in three TNBC cell lines. Western-blot was performed on 50 µg extracts from each TNBC cell line (MDA-MB-231, HCC-38 and MDA-MB-468) with antibodies directed against P-gp or ABCG2 and GAPDH. B. P-gp, ABCG2 and GAPDH signals were quantified using ImageJ software. Data presented are the mean ± SEM of at least 3 independent experiments. Significance is calculated using Oneway ANOVA Turkey’s multiple comparisons test and attained at P < 0.05 (*). ** P < 0.01; *** P < 0.001; ns P > 0.05.
    Figure Legend Snippet: Other multidrug transporters are expressed in TNBC cell lines. A. P-gp and ABCG2 protein expression in three TNBC cell lines. Western-blot was performed on 50 µg extracts from each TNBC cell line (MDA-MB-231, HCC-38 and MDA-MB-468) with antibodies directed against P-gp or ABCG2 and GAPDH. B. P-gp, ABCG2 and GAPDH signals were quantified using ImageJ software. Data presented are the mean ± SEM of at least 3 independent experiments. Significance is calculated using Oneway ANOVA Turkey’s multiple comparisons test and attained at P < 0.05 (*). ** P < 0.01; *** P < 0.001; ns P > 0.05.

    Techniques Used: Expressing, Western Blot, Software

    PAH increases docetaxel efficacy against TNBC spheroids. MDA-MB-231 cells were plated in ultra-low attachment surface 24 well plates in complete medium and treated with increasing concentrations of docetaxel in the presence of DMSO (control, 0 PAH), PAH 10 µM or 30 µM. After 2 weeks pictures were taken using Cytation 5 cell imaging system from Biotek. The surface of spheroids was calculated and reported after normalization on the condition without docetaxel for each concentration of PAH.
    Figure Legend Snippet: PAH increases docetaxel efficacy against TNBC spheroids. MDA-MB-231 cells were plated in ultra-low attachment surface 24 well plates in complete medium and treated with increasing concentrations of docetaxel in the presence of DMSO (control, 0 PAH), PAH 10 µM or 30 µM. After 2 weeks pictures were taken using Cytation 5 cell imaging system from Biotek. The surface of spheroids was calculated and reported after normalization on the condition without docetaxel for each concentration of PAH.

    Techniques Used: Control, Imaging, Concentration Assay

    PAH addition to chemotherapy inhibits migration of TNBC cells. A. 100000 cells were seeded on membrane from Transwell plates. Cells were treated 24 h with doxorubicin ± 15µM PAH or 48 h with docetaxel ± 15µM PAH. After fixation and staining with crystal violet, wells were observed on a microscope with X5 objective. B. Migration was measured using a wound-healing assay. A wound was performed on MDA-MB-231 confluent cells seeded in 24 well plates. The medium was replaced by fresh medium containing 5 µM docetaxel in the absence of PAH, or the presence of 5 µM PAH. Two pictures were taken at two different points of each well immediately after wound, and 3 days after wound with 5X objective. The width of the wound was measured using ImageJ software and reported as final wound width/initial wound width in percentage. Data presented are the mean ± SEM of 3 independent experiments. Significance is attained at P < 0.05.
    Figure Legend Snippet: PAH addition to chemotherapy inhibits migration of TNBC cells. A. 100000 cells were seeded on membrane from Transwell plates. Cells were treated 24 h with doxorubicin ± 15µM PAH or 48 h with docetaxel ± 15µM PAH. After fixation and staining with crystal violet, wells were observed on a microscope with X5 objective. B. Migration was measured using a wound-healing assay. A wound was performed on MDA-MB-231 confluent cells seeded in 24 well plates. The medium was replaced by fresh medium containing 5 µM docetaxel in the absence of PAH, or the presence of 5 µM PAH. Two pictures were taken at two different points of each well immediately after wound, and 3 days after wound with 5X objective. The width of the wound was measured using ImageJ software and reported as final wound width/initial wound width in percentage. Data presented are the mean ± SEM of 3 independent experiments. Significance is attained at P < 0.05.

    Techniques Used: Migration, Membrane, Staining, Microscopy, Wound Healing Assay, Software

    PTCH1 contributes to the efflux of doxorubicin from TNBC cells. A. PTCH1 protein expression (left panel) and intracellular doxorubicin (right panel) were analyzed 16 h after transfection of MDA-MB-231 cells with PTCH1-siRNA or negative-control-siRNA. PTCH1 and GAPDH western blot signals were quantified using ImageJ software (left panel). For doxorubicin accumulation measurements (right panel), MDA-MB-231 cells were grown on slides and transfected with PTCH1-siRNA or negative-control-siRNA. After incubation with 10 µM doxorubicin, 3 coverslips were fixed for doxorubicin loading control; the other coverslips were incubated with efflux buffer and fixed. B. Docetaxel inhibits the accumulation of doxorubicin in MDA-MB-231 cells. Cells on coverslip were incubated with 10 µM doxorubicin or 10 µM doxorubicin and 50 µM docetaxel. Doxorubicin fluorescence images were acquired by epifluorescence microscopy using a 40X objective, and doxorubicin fluorescence was quantified using ImageJ software for about 100 cells per condition per experiment. Histograms are the mean ± SEM of 3 independent experiments. Significance is attained at P < 0.05.
    Figure Legend Snippet: PTCH1 contributes to the efflux of doxorubicin from TNBC cells. A. PTCH1 protein expression (left panel) and intracellular doxorubicin (right panel) were analyzed 16 h after transfection of MDA-MB-231 cells with PTCH1-siRNA or negative-control-siRNA. PTCH1 and GAPDH western blot signals were quantified using ImageJ software (left panel). For doxorubicin accumulation measurements (right panel), MDA-MB-231 cells were grown on slides and transfected with PTCH1-siRNA or negative-control-siRNA. After incubation with 10 µM doxorubicin, 3 coverslips were fixed for doxorubicin loading control; the other coverslips were incubated with efflux buffer and fixed. B. Docetaxel inhibits the accumulation of doxorubicin in MDA-MB-231 cells. Cells on coverslip were incubated with 10 µM doxorubicin or 10 µM doxorubicin and 50 µM docetaxel. Doxorubicin fluorescence images were acquired by epifluorescence microscopy using a 40X objective, and doxorubicin fluorescence was quantified using ImageJ software for about 100 cells per condition per experiment. Histograms are the mean ± SEM of 3 independent experiments. Significance is attained at P < 0.05.

    Techniques Used: Expressing, Transfection, Negative Control, Western Blot, Software, Incubation, Control, Fluorescence, Epifluorescence Microscopy

    Related Articles

    Multiple Displacement Amplification:

    Article Title: Comparing Heparin and Heparin Mimetics in Targeting Immunomodulatory Proteins from Platelets to Activate T Cell-Dependent Immune Response in Oncology
    Article Snippet: Platelets play a crucial role in the hematogenous metastasis of cancer.. Beyond their mechanical function of forming a protective cloak around circulating tumor cells, which can be antagonized by heparin, activated platelets release various immunomodulatory components.. Based on a proteome profiling of releasates, we address the immunosuppressive activities of TGF-β1 and MMP2 to CD4+ and CD8+ T cells in several in vitro assays and compare heparin and synthetic heparin mimetics in targeting them to reverse immunosuppression of platelets in an oncological context.

    Article Title: Mitofusin-2 suppresses tumor immune escape through EGFR/STAT3-mediated PD-L1 transcription
    Article Snippet: .. The human lung cancer cell lines A549 (CCL-185, RRID:CVCL_0023), NCI-H1299(CRL-5803, RRID:CVCL_0060), HCC827(CRL-2868, RRID:CVCL_2063), NCI-H1703(CRL-5889,RRID:CVCL_1490) and NCI-H1975 (CRL-5908, RRID:CVCL_1511), the human kidney cell line 786-O(CRL-1932, RRID:CVCL_1051), 769-P(CRL-1933, RRID:CVCL_1050) and the human breast cancer cell line MDA-MB-231(CRM-HTB-26, RRID:CVCL_0062) were purchased from ATCC (USA). .. OS-RC-2(CL-0177, RRID:CVCL_1626) and PC-9(CL-0668, RRID:CVCL_B260) were purchased from Procell (Wuhan, China), the mouse lung cancer cell line LL/2 (LLC1)(C5094, RRID: CVCL_4358) and the mouse kidney cancer cell line RENCA (C5190; RRID:CVCL_2174) were purchased from the BDBIO (Hangzhou, China).

    Article Title: Fructose-1,6-bisphosphate couples glycolytic activity to cell adhesion
    Article Snippet: 2-Deoxyglucose (Merck) was diluted in PBS to a concentration of 1 M, stored at −20 °C and freshly diluted to a working concentration of 25 mM in each experiment. .. The following cell lines were obtained from the American Type Culture Collection (ATCC), which authenticated them via short tandem repeat profiling: the human sarcoma cell line U-2 OS (ATCC: HTB-96; female), the human embryonic kidney cell line HEK293T (ATCC: CRL-3216; sex not specified), the human breast cancer cell line MDA-MB-231 (ATCC: HTB-26; female) and the telomerase-immortalized human retinal pigment epithelium cell line hTERT RPE-1 (ATTC: CRL-4000; female). ..

    Article Title: Multi-Targeting Effects and Orchestrated Induction of Multiple Cell Death Modalities by Silver-Copper Alloy Nanoparticles Functionalized Drug Nanocrystals in Cancer Cells.
    Article Snippet: To combat multidrug resistance and cancer stem cell (CSC) persistence, we constructed a tumor-targeted nanoplatform integrating silver/copper alloy nanoparticles (Cu−Ag NPs) and camptothecin (CPT) nanocrystals for synergistic multimodal therapy.. The nanocomposite was fabricated by stepwise assembly of CPT nanocrystals, a polydopamine coating, and functionalization with Cu−Ag NPs plus a tumor-mitochondria dual-targeting peptide.. It exhibited a hydrodynamic diameter of ∼152.67 nm, high colloidal stability, favorable photothermal performance, and pH/NIRresponsive drug release.

    Article Title: Mitochondrial preservation through the PKA-DRP1 axis empowers natural killer cells to resist acidic stress and retain cytotoxicity.
    Article Snippet: .. The human NK cell line NK-92MI, the human breast cancer cell line MDA-MB-231, and the mouse breast cancer cell line 4T1 were purchased from the American Type Culture Collection (Manassas, VA, USA). .. NK-92MI cells were cultured in Minimum AR TIC LE IN PR ES S Essential Medium Alpha (Gibco/Life Technologies, Grand Island, NY, USA) supplemented with 2 mM L-glutamine (Gibco/Life Technologies), 12.5% fetal bovine serum (Gibco/Life Technologies), 0.1 mM 2-mercaptoethanol (Gibco/Life Technologies), 1% penicillin/streptomycin (Gibco/Life Technologies), 0.02 mM folic acid (Sigma-Aldrich, St. Louis, MO, USA), and 0.2 mM inositol (Sigma-Aldrich).

    Article Title: Mitofusin-2 suppresses tumor immune escape through EGFR/STAT3-mediated PD-L1 transcription.
    Article Snippet: .. Cell lines The human lung cancer cell lines A549 (CCL-185, RRID:CVCL_0023), NCI-H1299(CRL-5803, RRID:CVCL_0060), HCC827(CRL-2868, RRID:CVCL_2063), NCI-H1703( CRL5889,RRID:CVCL_1490) and NCI-H1975 (CRL-5908, RRID:CVCL_1511), the human kidney cell line 786-O(CRL-1932, RRID:CVCL_1051), 769-P(CRL-1933, RRID:CVCL_1050) and the human breast cancer cell line MDA-MB-231(CRM-HTB-26, RRID:CVCL_0062) were purchased from ATCC (USA). .. OS-RC-2( CL-0177, RRID:CVCL_1626) and PC-9(CL-0668, RRID:CVCL_B260) were purchased from Procell (Wuhan China), the mouse lung cancer cell line LL/2 (LLC1)(C5094, RRID: CVCL_4358) and the mouse kidney cancer cell line RENCA (C5190; RRID:CVCL_2174) were purchased from the BDBIO (Hangzhou, China).

    Article Title: Fructose-1,6-bisphosphate couples glycolytic activity to cell adhesion.
    Article Snippet: 2-Deoxyglucose (Merck) was diluted in PBS to a concentration of 1 M, stored at −20 °C and freshly diluted to a working concentration of 25 mM in each experiment. .. The following cell lines were obtained from the American Type Culture Collection (ATCC), which authenticated them via short tandem repeat profiling: the human sarcoma cell line U-2 OS (ATCC: HTB-96; female), the human embryonic kidney cell line HEK293T (ATCC: CRL3216; sex not specified), the human breast cancer cell line MDA-MB-231 (ATCC: HTB-26; female) and the telomerase-immortalized human retinal pigment epithelium cell line hTERT RPE-1 (ATTC: CRL-4000; female). ..

    Cell Culture:

    Article Title: Comparing Heparin and Heparin Mimetics in Targeting Immunomodulatory Proteins from Platelets to Activate T Cell-Dependent Immune Response in Oncology
    Article Snippet: Platelets play a crucial role in the hematogenous metastasis of cancer.. Beyond their mechanical function of forming a protective cloak around circulating tumor cells, which can be antagonized by heparin, activated platelets release various immunomodulatory components.. Based on a proteome profiling of releasates, we address the immunosuppressive activities of TGF-β1 and MMP2 to CD4+ and CD8+ T cells in several in vitro assays and compare heparin and synthetic heparin mimetics in targeting them to reverse immunosuppression of platelets in an oncological context.

    Article Title: Multi-Targeting Effects and Orchestrated Induction of Multiple Cell Death Modalities by Silver-Copper Alloy Nanoparticles Functionalized Drug Nanocrystals in Cancer Cells.
    Article Snippet: To combat multidrug resistance and cancer stem cell (CSC) persistence, we constructed a tumor-targeted nanoplatform integrating silver/copper alloy nanoparticles (Cu−Ag NPs) and camptothecin (CPT) nanocrystals for synergistic multimodal therapy.. The nanocomposite was fabricated by stepwise assembly of CPT nanocrystals, a polydopamine coating, and functionalization with Cu−Ag NPs plus a tumor-mitochondria dual-targeting peptide.. It exhibited a hydrodynamic diameter of ∼152.67 nm, high colloidal stability, favorable photothermal performance, and pH/NIRresponsive drug release.

    Article Title: Paclitaxel drives TREM2 + macrophage expansion underlying its inferior therapeutic efficacy compared to Nab-paclitaxel
    Article Snippet: Human breast cancer cell line BT549 (ATCC, HTB-122), Mouse breast cancer cell line 4T1-Luc (ATCC, CRL-2539-LUC2) and human macrophage cell lines THP1 (ATCC, TIB-202) were cultured in RPMI-1640 medium. .. Human breast cancer cell line MDA-MB-231(ATCC, CRM-HTB-26) was cultured in L15 medium. .. Mouse breast cancer cell line Py8119 (ATCC, CRL-3278) was cultured in Ham’s F12K medium.

    Modification:

    Article Title: Comparing Heparin and Heparin Mimetics in Targeting Immunomodulatory Proteins from Platelets to Activate T Cell-Dependent Immune Response in Oncology
    Article Snippet: Platelets play a crucial role in the hematogenous metastasis of cancer.. Beyond their mechanical function of forming a protective cloak around circulating tumor cells, which can be antagonized by heparin, activated platelets release various immunomodulatory components.. Based on a proteome profiling of releasates, we address the immunosuppressive activities of TGF-β1 and MMP2 to CD4+ and CD8+ T cells in several in vitro assays and compare heparin and synthetic heparin mimetics in targeting them to reverse immunosuppression of platelets in an oncological context.

    MTT Assay:

    Article Title: Multi-Targeting Effects and Orchestrated Induction of Multiple Cell Death Modalities by Silver-Copper Alloy Nanoparticles Functionalized Drug Nanocrystals in Cancer Cells.
    Article Snippet: To combat multidrug resistance and cancer stem cell (CSC) persistence, we constructed a tumor-targeted nanoplatform integrating silver/copper alloy nanoparticles (Cu−Ag NPs) and camptothecin (CPT) nanocrystals for synergistic multimodal therapy.. The nanocomposite was fabricated by stepwise assembly of CPT nanocrystals, a polydopamine coating, and functionalization with Cu−Ag NPs plus a tumor-mitochondria dual-targeting peptide.. It exhibited a hydrodynamic diameter of ∼152.67 nm, high colloidal stability, favorable photothermal performance, and pH/NIRresponsive drug release.

    Activity Assay:

    Article Title: Multi-Targeting Effects and Orchestrated Induction of Multiple Cell Death Modalities by Silver-Copper Alloy Nanoparticles Functionalized Drug Nanocrystals in Cancer Cells.
    Article Snippet: To combat multidrug resistance and cancer stem cell (CSC) persistence, we constructed a tumor-targeted nanoplatform integrating silver/copper alloy nanoparticles (Cu−Ag NPs) and camptothecin (CPT) nanocrystals for synergistic multimodal therapy.. The nanocomposite was fabricated by stepwise assembly of CPT nanocrystals, a polydopamine coating, and functionalization with Cu−Ag NPs plus a tumor-mitochondria dual-targeting peptide.. It exhibited a hydrodynamic diameter of ∼152.67 nm, high colloidal stability, favorable photothermal performance, and pH/NIRresponsive drug release.

    Staining:

    Article Title: Multi-Targeting Effects and Orchestrated Induction of Multiple Cell Death Modalities by Silver-Copper Alloy Nanoparticles Functionalized Drug Nanocrystals in Cancer Cells.
    Article Snippet: To combat multidrug resistance and cancer stem cell (CSC) persistence, we constructed a tumor-targeted nanoplatform integrating silver/copper alloy nanoparticles (Cu−Ag NPs) and camptothecin (CPT) nanocrystals for synergistic multimodal therapy.. The nanocomposite was fabricated by stepwise assembly of CPT nanocrystals, a polydopamine coating, and functionalization with Cu−Ag NPs plus a tumor-mitochondria dual-targeting peptide.. It exhibited a hydrodynamic diameter of ∼152.67 nm, high colloidal stability, favorable photothermal performance, and pH/NIRresponsive drug release.

    TUNEL Assay:

    Article Title: Multi-Targeting Effects and Orchestrated Induction of Multiple Cell Death Modalities by Silver-Copper Alloy Nanoparticles Functionalized Drug Nanocrystals in Cancer Cells.
    Article Snippet: To combat multidrug resistance and cancer stem cell (CSC) persistence, we constructed a tumor-targeted nanoplatform integrating silver/copper alloy nanoparticles (Cu−Ag NPs) and camptothecin (CPT) nanocrystals for synergistic multimodal therapy.. The nanocomposite was fabricated by stepwise assembly of CPT nanocrystals, a polydopamine coating, and functionalization with Cu−Ag NPs plus a tumor-mitochondria dual-targeting peptide.. It exhibited a hydrodynamic diameter of ∼152.67 nm, high colloidal stability, favorable photothermal performance, and pH/NIRresponsive drug release.

    ATP Assay:

    Article Title: Multi-Targeting Effects and Orchestrated Induction of Multiple Cell Death Modalities by Silver-Copper Alloy Nanoparticles Functionalized Drug Nanocrystals in Cancer Cells.
    Article Snippet: To combat multidrug resistance and cancer stem cell (CSC) persistence, we constructed a tumor-targeted nanoplatform integrating silver/copper alloy nanoparticles (Cu−Ag NPs) and camptothecin (CPT) nanocrystals for synergistic multimodal therapy.. The nanocomposite was fabricated by stepwise assembly of CPT nanocrystals, a polydopamine coating, and functionalization with Cu−Ag NPs plus a tumor-mitochondria dual-targeting peptide.. It exhibited a hydrodynamic diameter of ∼152.67 nm, high colloidal stability, favorable photothermal performance, and pH/NIRresponsive drug release.

    Western Blot:

    Article Title: Multi-Targeting Effects and Orchestrated Induction of Multiple Cell Death Modalities by Silver-Copper Alloy Nanoparticles Functionalized Drug Nanocrystals in Cancer Cells.
    Article Snippet: To combat multidrug resistance and cancer stem cell (CSC) persistence, we constructed a tumor-targeted nanoplatform integrating silver/copper alloy nanoparticles (Cu−Ag NPs) and camptothecin (CPT) nanocrystals for synergistic multimodal therapy.. The nanocomposite was fabricated by stepwise assembly of CPT nanocrystals, a polydopamine coating, and functionalization with Cu−Ag NPs plus a tumor-mitochondria dual-targeting peptide.. It exhibited a hydrodynamic diameter of ∼152.67 nm, high colloidal stability, favorable photothermal performance, and pH/NIRresponsive drug release.

    Bioprocessing:

    Article Title: Multi-Targeting Effects and Orchestrated Induction of Multiple Cell Death Modalities by Silver-Copper Alloy Nanoparticles Functionalized Drug Nanocrystals in Cancer Cells.
    Article Snippet: To combat multidrug resistance and cancer stem cell (CSC) persistence, we constructed a tumor-targeted nanoplatform integrating silver/copper alloy nanoparticles (Cu−Ag NPs) and camptothecin (CPT) nanocrystals for synergistic multimodal therapy.. The nanocomposite was fabricated by stepwise assembly of CPT nanocrystals, a polydopamine coating, and functionalization with Cu−Ag NPs plus a tumor-mitochondria dual-targeting peptide.. It exhibited a hydrodynamic diameter of ∼152.67 nm, high colloidal stability, favorable photothermal performance, and pH/NIRresponsive drug release.

    Synthesized:

    Article Title: Multi-Targeting Effects and Orchestrated Induction of Multiple Cell Death Modalities by Silver-Copper Alloy Nanoparticles Functionalized Drug Nanocrystals in Cancer Cells.
    Article Snippet: To combat multidrug resistance and cancer stem cell (CSC) persistence, we constructed a tumor-targeted nanoplatform integrating silver/copper alloy nanoparticles (Cu−Ag NPs) and camptothecin (CPT) nanocrystals for synergistic multimodal therapy.. The nanocomposite was fabricated by stepwise assembly of CPT nanocrystals, a polydopamine coating, and functionalization with Cu−Ag NPs plus a tumor-mitochondria dual-targeting peptide.. It exhibited a hydrodynamic diameter of ∼152.67 nm, high colloidal stability, favorable photothermal performance, and pH/NIRresponsive drug release.



    Similar Products

    human breast cancer cell lines mda mb 231  (ATCC)
    99
    ATCC human breast cancer cell lines mda mb 231
    Ptch1 is expressed in breast cancer cell lines. A. PTCH1 mRNA expression (log 2 transformed) in various luminal and HER2 breast cancer cell lines (dark green) and in TNBC cell lines (other colors). TNBC cell lines are depicted according to the “Lehmann TNBC subtype” nomenclature : basal-like 1 (yellow), basal-like 2 (pale green), immunomodulatory (brown), luminal androgen receptor (dark pink), mesenchymal (pale pink) and mesenchymal stem-like (pink). B. PTCH1 protein expression in three TNBC cell lines. Western-blot was performed on 50 µg extracts from TNBC cell lines <t>(MDA-MB-231,</t> HCC-38 and MDA-MB-468) with antibodies directed against PTCH1. PTCH1 and GAPDH signals were quantified using ImageJ software. Data presented are the mean ± SEM of at least 3 independent experiments. Significance is calculated using Oneway ANOVA Turkey’s multiple comparisons test and attained at P < 0.05 (*).
    Human Breast Cancer Cell Lines Mda Mb 231, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+breast+cancer+cell+line+mda+mb+231/MDA-MB-468/pmc13101699-47-0-12
    Average 99 stars, based on 1 article reviews
    human breast cancer cell lines mda mb 231 - by Bioz Stars, 2026-09
    99/100 stars
    		  Buy from Supplier
    cell lines 455 human breast cancer cells mda mb 231  (ATCC)
    99
    ATCC cell lines 455 human breast cancer cells mda mb 231
    Ptch1 is expressed in breast cancer cell lines. A. PTCH1 mRNA expression (log 2 transformed) in various luminal and HER2 breast cancer cell lines (dark green) and in TNBC cell lines (other colors). TNBC cell lines are depicted according to the “Lehmann TNBC subtype” nomenclature : basal-like 1 (yellow), basal-like 2 (pale green), immunomodulatory (brown), luminal androgen receptor (dark pink), mesenchymal (pale pink) and mesenchymal stem-like (pink). B. PTCH1 protein expression in three TNBC cell lines. Western-blot was performed on 50 µg extracts from TNBC cell lines <t>(MDA-MB-231,</t> HCC-38 and MDA-MB-468) with antibodies directed against PTCH1. PTCH1 and GAPDH signals were quantified using ImageJ software. Data presented are the mean ± SEM of at least 3 independent experiments. Significance is calculated using Oneway ANOVA Turkey’s multiple comparisons test and attained at P < 0.05 (*).
    Cell Lines 455 Human Breast Cancer Cells Mda Mb 231, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+breast+cancer+cell+line+mda+mb+231/MDA-MB-231/pm42162647-221-0-8
    Average 99 stars, based on 1 article reviews
    cell lines 455 human breast cancer cells mda mb 231 - by Bioz Stars, 2026-09
    99/100 stars
    		  Buy from Supplier
    breast cancer tnbc cell lines human mda mb 231  (ATCC)
    99
    ATCC breast cancer tnbc cell lines human mda mb 231
    Cell viability of the cell lines MCF-10A (A), MCF-7 <t>(B),</t> <t>and</t> <t>MDA-MB-231</t> (C) after treatments with IsCT1 (green bars) or AKFK-IsCT1 (purple bars) at concentrations up to 4.0 μM, followed by incubation at 37 °C and 5% CO 2 for 4 h. Green bars represent IsCT1, and pink bars represent AKFK-IsCT1. Data are expressed as mean ± SD of three independent experiments performed in triplicate. Statistical significance was determined using two-way ANOVA followed by Tukey test, ** p < 0.005; **** p < 0.0001.
    Breast Cancer Tnbc Cell Lines Human Mda Mb 231, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+breast+cancer+cell+line+mda+mb+231/MDA-MB-231/pmc13270443-122-2-32
    Average 99 stars, based on 1 article reviews
    breast cancer tnbc cell lines human mda mb 231 - by Bioz Stars, 2026-09
    99/100 stars
    		  Buy from Supplier
    human breast cancer metastatic cell line  (ATCC)
    99
    ATCC human breast cancer metastatic cell line
    Cell viability of the cell lines MCF-10A (A), MCF-7 <t>(B),</t> <t>and</t> <t>MDA-MB-231</t> (C) after treatments with IsCT1 (green bars) or AKFK-IsCT1 (purple bars) at concentrations up to 4.0 μM, followed by incubation at 37 °C and 5% CO 2 for 4 h. Green bars represent IsCT1, and pink bars represent AKFK-IsCT1. Data are expressed as mean ± SD of three independent experiments performed in triplicate. Statistical significance was determined using two-way ANOVA followed by Tukey test, ** p < 0.005; **** p < 0.0001.
    Human Breast Cancer Metastatic Cell Line, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+breast+cancer+cell+line+mda+mb+231/MDA-MB-231/pmc13103895-46-0-11
    Average 99 stars, based on 1 article reviews
    human breast cancer metastatic cell line - by Bioz Stars, 2026-09
    99/100 stars
    		  Buy from Supplier
    culture conditions human breast cancer cell line mda mb  (ATCC)
    99
    ATCC culture conditions human breast cancer cell line mda mb
    Cell viability of the cell lines MCF-10A (A), MCF-7 <t>(B),</t> <t>and</t> <t>MDA-MB-231</t> (C) after treatments with IsCT1 (green bars) or AKFK-IsCT1 (purple bars) at concentrations up to 4.0 μM, followed by incubation at 37 °C and 5% CO 2 for 4 h. Green bars represent IsCT1, and pink bars represent AKFK-IsCT1. Data are expressed as mean ± SD of three independent experiments performed in triplicate. Statistical significance was determined using two-way ANOVA followed by Tukey test, ** p < 0.005; **** p < 0.0001.
    Culture Conditions Human Breast Cancer Cell Line Mda Mb, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+breast+cancer+cell+line+mda+mb+231/MDA-MB-231/us12595289-555-6-17
    Average 99 stars, based on 1 article reviews
    culture conditions human breast cancer cell line mda mb - by Bioz Stars, 2026-09
    99/100 stars
    		  Buy from Supplier
    culture conditions 568 human breast cancer cells lines mda mb 231  (ATCC)
    99
    ATCC culture conditions 568 human breast cancer cells lines mda mb 231
    Cell viability of the cell lines MCF-10A (A), MCF-7 <t>(B),</t> <t>and</t> <t>MDA-MB-231</t> (C) after treatments with IsCT1 (green bars) or AKFK-IsCT1 (purple bars) at concentrations up to 4.0 μM, followed by incubation at 37 °C and 5% CO 2 for 4 h. Green bars represent IsCT1, and pink bars represent AKFK-IsCT1. Data are expressed as mean ± SD of three independent experiments performed in triplicate. Statistical significance was determined using two-way ANOVA followed by Tukey test, ** p < 0.005; **** p < 0.0001.
    Culture Conditions 568 Human Breast Cancer Cells Lines Mda Mb 231, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+breast+cancer+cell+line+mda+mb+231/MDA-MB-231/10__1158_slash_2159___8290__cd___25___1459-268-5-36
    Average 99 stars, based on 1 article reviews
    culture conditions 568 human breast cancer cells lines mda mb 231 - by Bioz Stars, 2026-09
    99/100 stars
    		  Buy from Supplier
    human breast cancer cell line mda mb 231  (Procell Inc)
    86
    Procell Inc human breast cancer cell line mda mb 231
    Cell viability of the cell lines MCF-10A (A), MCF-7 <t>(B),</t> <t>and</t> <t>MDA-MB-231</t> (C) after treatments with IsCT1 (green bars) or AKFK-IsCT1 (purple bars) at concentrations up to 4.0 μM, followed by incubation at 37 °C and 5% CO 2 for 4 h. Green bars represent IsCT1, and pink bars represent AKFK-IsCT1. Data are expressed as mean ± SD of three independent experiments performed in triplicate. Statistical significance was determined using two-way ANOVA followed by Tukey test, ** p < 0.005; **** p < 0.0001.
    Human Breast Cancer Cell Line Mda Mb 231, supplied by Procell Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+breast+cancer+cell+line+mda+mb+231/231+mb+mda/10__1016_slash_j__phymed__2026__158250-88-1-18
    Average 86 stars, based on 1 article reviews
    human breast cancer cell line mda mb 231 - by Bioz Stars, 2026-09
    86/100 stars
    		  Buy from Supplier
    cell lines human breast cancer cells mda mb 231 atcc  (ATCC)
    99
    ATCC cell lines human breast cancer cells mda mb 231 atcc
    Cell viability of the cell lines MCF-10A (A), MCF-7 <t>(B),</t> <t>and</t> <t>MDA-MB-231</t> (C) after treatments with IsCT1 (green bars) or AKFK-IsCT1 (purple bars) at concentrations up to 4.0 μM, followed by incubation at 37 °C and 5% CO 2 for 4 h. Green bars represent IsCT1, and pink bars represent AKFK-IsCT1. Data are expressed as mean ± SD of three independent experiments performed in triplicate. Statistical significance was determined using two-way ANOVA followed by Tukey test, ** p < 0.005; **** p < 0.0001.
    Cell Lines Human Breast Cancer Cells Mda Mb 231 Atcc, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+breast+cancer+cell+line+mda+mb+231/MDA-MB-231/pm41923644-663-100-107
    Average 99 stars, based on 1 article reviews
    cell lines human breast cancer cells mda mb 231 atcc - by Bioz Stars, 2026-09
    99/100 stars
    		  Buy from Supplier

    Image Search Results


    Ptch1 is expressed in breast cancer cell lines. A. PTCH1 mRNA expression (log 2 transformed) in various luminal and HER2 breast cancer cell lines (dark green) and in TNBC cell lines (other colors). TNBC cell lines are depicted according to the “Lehmann TNBC subtype” nomenclature : basal-like 1 (yellow), basal-like 2 (pale green), immunomodulatory (brown), luminal androgen receptor (dark pink), mesenchymal (pale pink) and mesenchymal stem-like (pink). B. PTCH1 protein expression in three TNBC cell lines. Western-blot was performed on 50 µg extracts from TNBC cell lines (MDA-MB-231, HCC-38 and MDA-MB-468) with antibodies directed against PTCH1. PTCH1 and GAPDH signals were quantified using ImageJ software. Data presented are the mean ± SEM of at least 3 independent experiments. Significance is calculated using Oneway ANOVA Turkey’s multiple comparisons test and attained at P < 0.05 (*).

    Journal: Translational Oncology

    Article Title: Inhibition of PTCH1 drug efflux activity enhances chemotherapy efficacy against triple negative breast cancers

    doi: 10.1016/j.tranon.2026.102777

    Figure Lengend Snippet: Ptch1 is expressed in breast cancer cell lines. A. PTCH1 mRNA expression (log 2 transformed) in various luminal and HER2 breast cancer cell lines (dark green) and in TNBC cell lines (other colors). TNBC cell lines are depicted according to the “Lehmann TNBC subtype” nomenclature : basal-like 1 (yellow), basal-like 2 (pale green), immunomodulatory (brown), luminal androgen receptor (dark pink), mesenchymal (pale pink) and mesenchymal stem-like (pink). B. PTCH1 protein expression in three TNBC cell lines. Western-blot was performed on 50 µg extracts from TNBC cell lines (MDA-MB-231, HCC-38 and MDA-MB-468) with antibodies directed against PTCH1. PTCH1 and GAPDH signals were quantified using ImageJ software. Data presented are the mean ± SEM of at least 3 independent experiments. Significance is calculated using Oneway ANOVA Turkey’s multiple comparisons test and attained at P < 0.05 (*).

    Article Snippet: Human breast cancer cell lines MDA-MB-231, MDA-MB-468 and HCC-38 were purchased from ATCC.

    Techniques: Expressing, Transformation Assay, Western Blot, Software

    PTCH1 drug efflux inhibitor PAH increases the sensitivity of TNBC cells to chemotherapy . Cell viability was measured after 24 h or 48 h treatment with increasing concentration of docetaxel or doxorubicin respectively on MDA-MB-231, MDA-MB-468 and HCC-38 cell lines in the absence or the presence of 15µM PAH. IC 50 values (corresponding to the concentration of chemotherapy inducing 50% of cell death) were calculated. Data reported are the mean ± SEM of at least 3 independent experiments. Significance is attained at P < 0.05 (*).

    Journal: Translational Oncology

    Article Title: Inhibition of PTCH1 drug efflux activity enhances chemotherapy efficacy against triple negative breast cancers

    doi: 10.1016/j.tranon.2026.102777

    Figure Lengend Snippet: PTCH1 drug efflux inhibitor PAH increases the sensitivity of TNBC cells to chemotherapy . Cell viability was measured after 24 h or 48 h treatment with increasing concentration of docetaxel or doxorubicin respectively on MDA-MB-231, MDA-MB-468 and HCC-38 cell lines in the absence or the presence of 15µM PAH. IC 50 values (corresponding to the concentration of chemotherapy inducing 50% of cell death) were calculated. Data reported are the mean ± SEM of at least 3 independent experiments. Significance is attained at P < 0.05 (*).

    Article Snippet: Human breast cancer cell lines MDA-MB-231, MDA-MB-468 and HCC-38 were purchased from ATCC.

    Techniques: Concentration Assay

    Other multidrug transporters are expressed in TNBC cell lines. A. P-gp and ABCG2 protein expression in three TNBC cell lines. Western-blot was performed on 50 µg extracts from each TNBC cell line (MDA-MB-231, HCC-38 and MDA-MB-468) with antibodies directed against P-gp or ABCG2 and GAPDH. B. P-gp, ABCG2 and GAPDH signals were quantified using ImageJ software. Data presented are the mean ± SEM of at least 3 independent experiments. Significance is calculated using Oneway ANOVA Turkey’s multiple comparisons test and attained at P < 0.05 (*). ** P < 0.01; *** P < 0.001; ns P > 0.05.

    Journal: Translational Oncology

    Article Title: Inhibition of PTCH1 drug efflux activity enhances chemotherapy efficacy against triple negative breast cancers

    doi: 10.1016/j.tranon.2026.102777

    Figure Lengend Snippet: Other multidrug transporters are expressed in TNBC cell lines. A. P-gp and ABCG2 protein expression in three TNBC cell lines. Western-blot was performed on 50 µg extracts from each TNBC cell line (MDA-MB-231, HCC-38 and MDA-MB-468) with antibodies directed against P-gp or ABCG2 and GAPDH. B. P-gp, ABCG2 and GAPDH signals were quantified using ImageJ software. Data presented are the mean ± SEM of at least 3 independent experiments. Significance is calculated using Oneway ANOVA Turkey’s multiple comparisons test and attained at P < 0.05 (*). ** P < 0.01; *** P < 0.001; ns P > 0.05.

    Article Snippet: Human breast cancer cell lines MDA-MB-231, MDA-MB-468 and HCC-38 were purchased from ATCC.

    Techniques: Expressing, Western Blot, Software

    PAH increases docetaxel efficacy against TNBC spheroids. MDA-MB-231 cells were plated in ultra-low attachment surface 24 well plates in complete medium and treated with increasing concentrations of docetaxel in the presence of DMSO (control, 0 PAH), PAH 10 µM or 30 µM. After 2 weeks pictures were taken using Cytation 5 cell imaging system from Biotek. The surface of spheroids was calculated and reported after normalization on the condition without docetaxel for each concentration of PAH.

    Journal: Translational Oncology

    Article Title: Inhibition of PTCH1 drug efflux activity enhances chemotherapy efficacy against triple negative breast cancers

    doi: 10.1016/j.tranon.2026.102777

    Figure Lengend Snippet: PAH increases docetaxel efficacy against TNBC spheroids. MDA-MB-231 cells were plated in ultra-low attachment surface 24 well plates in complete medium and treated with increasing concentrations of docetaxel in the presence of DMSO (control, 0 PAH), PAH 10 µM or 30 µM. After 2 weeks pictures were taken using Cytation 5 cell imaging system from Biotek. The surface of spheroids was calculated and reported after normalization on the condition without docetaxel for each concentration of PAH.

    Article Snippet: Human breast cancer cell lines MDA-MB-231, MDA-MB-468 and HCC-38 were purchased from ATCC.

    Techniques: Control, Imaging, Concentration Assay

    PAH addition to chemotherapy inhibits migration of TNBC cells. A. 100000 cells were seeded on membrane from Transwell plates. Cells were treated 24 h with doxorubicin ± 15µM PAH or 48 h with docetaxel ± 15µM PAH. After fixation and staining with crystal violet, wells were observed on a microscope with X5 objective. B. Migration was measured using a wound-healing assay. A wound was performed on MDA-MB-231 confluent cells seeded in 24 well plates. The medium was replaced by fresh medium containing 5 µM docetaxel in the absence of PAH, or the presence of 5 µM PAH. Two pictures were taken at two different points of each well immediately after wound, and 3 days after wound with 5X objective. The width of the wound was measured using ImageJ software and reported as final wound width/initial wound width in percentage. Data presented are the mean ± SEM of 3 independent experiments. Significance is attained at P < 0.05.

    Journal: Translational Oncology

    Article Title: Inhibition of PTCH1 drug efflux activity enhances chemotherapy efficacy against triple negative breast cancers

    doi: 10.1016/j.tranon.2026.102777

    Figure Lengend Snippet: PAH addition to chemotherapy inhibits migration of TNBC cells. A. 100000 cells were seeded on membrane from Transwell plates. Cells were treated 24 h with doxorubicin ± 15µM PAH or 48 h with docetaxel ± 15µM PAH. After fixation and staining with crystal violet, wells were observed on a microscope with X5 objective. B. Migration was measured using a wound-healing assay. A wound was performed on MDA-MB-231 confluent cells seeded in 24 well plates. The medium was replaced by fresh medium containing 5 µM docetaxel in the absence of PAH, or the presence of 5 µM PAH. Two pictures were taken at two different points of each well immediately after wound, and 3 days after wound with 5X objective. The width of the wound was measured using ImageJ software and reported as final wound width/initial wound width in percentage. Data presented are the mean ± SEM of 3 independent experiments. Significance is attained at P < 0.05.

    Article Snippet: Human breast cancer cell lines MDA-MB-231, MDA-MB-468 and HCC-38 were purchased from ATCC.

    Techniques: Migration, Membrane, Staining, Microscopy, Wound Healing Assay, Software

    PTCH1 contributes to the efflux of doxorubicin from TNBC cells. A. PTCH1 protein expression (left panel) and intracellular doxorubicin (right panel) were analyzed 16 h after transfection of MDA-MB-231 cells with PTCH1-siRNA or negative-control-siRNA. PTCH1 and GAPDH western blot signals were quantified using ImageJ software (left panel). For doxorubicin accumulation measurements (right panel), MDA-MB-231 cells were grown on slides and transfected with PTCH1-siRNA or negative-control-siRNA. After incubation with 10 µM doxorubicin, 3 coverslips were fixed for doxorubicin loading control; the other coverslips were incubated with efflux buffer and fixed. B. Docetaxel inhibits the accumulation of doxorubicin in MDA-MB-231 cells. Cells on coverslip were incubated with 10 µM doxorubicin or 10 µM doxorubicin and 50 µM docetaxel. Doxorubicin fluorescence images were acquired by epifluorescence microscopy using a 40X objective, and doxorubicin fluorescence was quantified using ImageJ software for about 100 cells per condition per experiment. Histograms are the mean ± SEM of 3 independent experiments. Significance is attained at P < 0.05.

    Journal: Translational Oncology

    Article Title: Inhibition of PTCH1 drug efflux activity enhances chemotherapy efficacy against triple negative breast cancers

    doi: 10.1016/j.tranon.2026.102777

    Figure Lengend Snippet: PTCH1 contributes to the efflux of doxorubicin from TNBC cells. A. PTCH1 protein expression (left panel) and intracellular doxorubicin (right panel) were analyzed 16 h after transfection of MDA-MB-231 cells with PTCH1-siRNA or negative-control-siRNA. PTCH1 and GAPDH western blot signals were quantified using ImageJ software (left panel). For doxorubicin accumulation measurements (right panel), MDA-MB-231 cells were grown on slides and transfected with PTCH1-siRNA or negative-control-siRNA. After incubation with 10 µM doxorubicin, 3 coverslips were fixed for doxorubicin loading control; the other coverslips were incubated with efflux buffer and fixed. B. Docetaxel inhibits the accumulation of doxorubicin in MDA-MB-231 cells. Cells on coverslip were incubated with 10 µM doxorubicin or 10 µM doxorubicin and 50 µM docetaxel. Doxorubicin fluorescence images were acquired by epifluorescence microscopy using a 40X objective, and doxorubicin fluorescence was quantified using ImageJ software for about 100 cells per condition per experiment. Histograms are the mean ± SEM of 3 independent experiments. Significance is attained at P < 0.05.

    Article Snippet: Human breast cancer cell lines MDA-MB-231, MDA-MB-468 and HCC-38 were purchased from ATCC.

    Techniques: Expressing, Transfection, Negative Control, Western Blot, Software, Incubation, Control, Fluorescence, Epifluorescence Microscopy

    Cell viability of the cell lines MCF-10A (A), MCF-7 (B), and MDA-MB-231 (C) after treatments with IsCT1 (green bars) or AKFK-IsCT1 (purple bars) at concentrations up to 4.0 μM, followed by incubation at 37 °C and 5% CO 2 for 4 h. Green bars represent IsCT1, and pink bars represent AKFK-IsCT1. Data are expressed as mean ± SD of three independent experiments performed in triplicate. Statistical significance was determined using two-way ANOVA followed by Tukey test, ** p < 0.005; **** p < 0.0001.

    Journal: ACS Pharmacology & Translational Science

    Article Title: Engineering the Host Defense Peptide from Scorpion Venom for Safer and More Potent Antibreast Cancer Activity

    doi: 10.1021/acsptsci.5c00598

    Figure Lengend Snippet: Cell viability of the cell lines MCF-10A (A), MCF-7 (B), and MDA-MB-231 (C) after treatments with IsCT1 (green bars) or AKFK-IsCT1 (purple bars) at concentrations up to 4.0 μM, followed by incubation at 37 °C and 5% CO 2 for 4 h. Green bars represent IsCT1, and pink bars represent AKFK-IsCT1. Data are expressed as mean ± SD of three independent experiments performed in triplicate. Statistical significance was determined using two-way ANOVA followed by Tukey test, ** p < 0.005; **** p < 0.0001.

    Article Snippet: The triple-negative breast cancer (TNBC) cell lines human MDA-MB-231 (CRM-HTB-26) and murine 4T1 (CRL-2539), human adenocarcinoma cell line MCF-7 (HTB-22), and the human breast epithelial cell line MCF-10A (CRL-10317) were obtained from American Type Culture Collection (ATCC).

    Techniques: Incubation

    Cell viability after combined treatment with photodynamic therapy using hypericin (PDT-Hyp, 0.05 μM) and peptides under two treatment protocols. Protocol 1 (PDT-Hyp followed by peptide exposure) was applied to (A) MCF-10A, (B) MCF-7, and (C) MDA-MB-231 cells, whereas Protocol 2 (peptide exposure followed by PDT-Hyp) was applied to (D) MCF-10A, (E) MCF-7, and (F) MDA-MB-231 cells. Blue bars represent IsCT1, and red bars represent AKFK-IsCT1. Peptides were tested at concentrations ranging from 0.01 to 4.0 μM. Cells were incubated at 37 °C and 5% CO 2 for 4 h following treatment. Data are expressed as mean ± SD of three independent experiments performed in triplicate. Statistical significance was determined using two-way ANOVA followed by Tukey test, * p < 0.05; ** p < 0.005; *** p < 0.0005; **** p < 0.0001.

    Journal: ACS Pharmacology & Translational Science

    Article Title: Engineering the Host Defense Peptide from Scorpion Venom for Safer and More Potent Antibreast Cancer Activity

    doi: 10.1021/acsptsci.5c00598

    Figure Lengend Snippet: Cell viability after combined treatment with photodynamic therapy using hypericin (PDT-Hyp, 0.05 μM) and peptides under two treatment protocols. Protocol 1 (PDT-Hyp followed by peptide exposure) was applied to (A) MCF-10A, (B) MCF-7, and (C) MDA-MB-231 cells, whereas Protocol 2 (peptide exposure followed by PDT-Hyp) was applied to (D) MCF-10A, (E) MCF-7, and (F) MDA-MB-231 cells. Blue bars represent IsCT1, and red bars represent AKFK-IsCT1. Peptides were tested at concentrations ranging from 0.01 to 4.0 μM. Cells were incubated at 37 °C and 5% CO 2 for 4 h following treatment. Data are expressed as mean ± SD of three independent experiments performed in triplicate. Statistical significance was determined using two-way ANOVA followed by Tukey test, * p < 0.05; ** p < 0.005; *** p < 0.0005; **** p < 0.0001.

    Article Snippet: The triple-negative breast cancer (TNBC) cell lines human MDA-MB-231 (CRM-HTB-26) and murine 4T1 (CRL-2539), human adenocarcinoma cell line MCF-7 (HTB-22), and the human breast epithelial cell line MCF-10A (CRL-10317) were obtained from American Type Culture Collection (ATCC).

    Techniques: Incubation

    Three-dimensional Bliss synergy response surfaces for the combined treatment of PDT-Hyp and peptides applied under different treatment protocols. Protocol 1 was evaluated with IsCT1 (A, E, I) or AKFK-IsCT1 (C, G, K), whereas Protocol 2 was evaluated with IsCT1 (B, F, J) or AKFK-IsCT1 (D, H, L). Panels in the first row (A–D) correspond to MCF-10A cells, the second row (E–H) to MCF-7 cells, and the third row (I–L) to MDA-MB-231 cells. Color coding represents Bliss synergy scores, where green indicates antagonism (≤0), intermediate colors between white and light red indicate additive effects (>0 and ≤10), and red indicates synergistic interactions (>10). Color intensity is proportional to the magnitude of the interaction score. Bliss synergy scores and response surfaces were generated from complete dose–response matrices using SynergyFinder 3.0.

    Journal: ACS Pharmacology & Translational Science

    Article Title: Engineering the Host Defense Peptide from Scorpion Venom for Safer and More Potent Antibreast Cancer Activity

    doi: 10.1021/acsptsci.5c00598

    Figure Lengend Snippet: Three-dimensional Bliss synergy response surfaces for the combined treatment of PDT-Hyp and peptides applied under different treatment protocols. Protocol 1 was evaluated with IsCT1 (A, E, I) or AKFK-IsCT1 (C, G, K), whereas Protocol 2 was evaluated with IsCT1 (B, F, J) or AKFK-IsCT1 (D, H, L). Panels in the first row (A–D) correspond to MCF-10A cells, the second row (E–H) to MCF-7 cells, and the third row (I–L) to MDA-MB-231 cells. Color coding represents Bliss synergy scores, where green indicates antagonism (≤0), intermediate colors between white and light red indicate additive effects (>0 and ≤10), and red indicates synergistic interactions (>10). Color intensity is proportional to the magnitude of the interaction score. Bliss synergy scores and response surfaces were generated from complete dose–response matrices using SynergyFinder 3.0.

    Article Snippet: The triple-negative breast cancer (TNBC) cell lines human MDA-MB-231 (CRM-HTB-26) and murine 4T1 (CRL-2539), human adenocarcinoma cell line MCF-7 (HTB-22), and the human breast epithelial cell line MCF-10A (CRL-10317) were obtained from American Type Culture Collection (ATCC).

    Techniques: Generated