human breast cancer cell lines mda mb 231  (ATCC)

Journal: Translational Oncology

Article Title: Inhibition of PTCH1 drug efflux activity enhances chemotherapy efficacy against triple negative breast cancers

doi: 10.1016/j.tranon.2026.102777

Figure Lengend Snippet: Ptch1 is expressed in breast cancer cell lines. A. PTCH1 mRNA expression (log 2 transformed) in various luminal and HER2 breast cancer cell lines (dark green) and in TNBC cell lines (other colors). TNBC cell lines are depicted according to the “Lehmann TNBC subtype” nomenclature : basal-like 1 (yellow), basal-like 2 (pale green), immunomodulatory (brown), luminal androgen receptor (dark pink), mesenchymal (pale pink) and mesenchymal stem-like (pink). B. PTCH1 protein expression in three TNBC cell lines. Western-blot was performed on 50 µg extracts from TNBC cell lines (MDA-MB-231, HCC-38 and MDA-MB-468) with antibodies directed against PTCH1. PTCH1 and GAPDH signals were quantified using ImageJ software. Data presented are the mean ± SEM of at least 3 independent experiments. Significance is calculated using Oneway ANOVA Turkey’s multiple comparisons test and attained at P < 0.05 (*).

Article Snippet: Human breast cancer cell lines MDA-MB-231, MDA-MB-468 and HCC-38 were purchased from ATCC.

Techniques: Expressing, Transformation Assay, Western Blot, Software

Journal: Translational Oncology

Article Title: Inhibition of PTCH1 drug efflux activity enhances chemotherapy efficacy against triple negative breast cancers

doi: 10.1016/j.tranon.2026.102777

Figure Lengend Snippet: PTCH1 drug efflux inhibitor PAH increases the sensitivity of TNBC cells to chemotherapy . Cell viability was measured after 24 h or 48 h treatment with increasing concentration of docetaxel or doxorubicin respectively on MDA-MB-231, MDA-MB-468 and HCC-38 cell lines in the absence or the presence of 15µM PAH. IC 50 values (corresponding to the concentration of chemotherapy inducing 50% of cell death) were calculated. Data reported are the mean ± SEM of at least 3 independent experiments. Significance is attained at P < 0.05 (*).

Article Snippet: Human breast cancer cell lines MDA-MB-231, MDA-MB-468 and HCC-38 were purchased from ATCC.

Techniques: Concentration Assay

Journal: Translational Oncology

Article Title: Inhibition of PTCH1 drug efflux activity enhances chemotherapy efficacy against triple negative breast cancers

doi: 10.1016/j.tranon.2026.102777

Figure Lengend Snippet: Other multidrug transporters are expressed in TNBC cell lines. A. P-gp and ABCG2 protein expression in three TNBC cell lines. Western-blot was performed on 50 µg extracts from each TNBC cell line (MDA-MB-231, HCC-38 and MDA-MB-468) with antibodies directed against P-gp or ABCG2 and GAPDH. B. P-gp, ABCG2 and GAPDH signals were quantified using ImageJ software. Data presented are the mean ± SEM of at least 3 independent experiments. Significance is calculated using Oneway ANOVA Turkey’s multiple comparisons test and attained at P < 0.05 (*). ** P < 0.01; *** P < 0.001; ns P > 0.05.

Article Snippet: Human breast cancer cell lines MDA-MB-231, MDA-MB-468 and HCC-38 were purchased from ATCC.

Techniques: Expressing, Western Blot, Software

Journal: Translational Oncology

Article Title: Inhibition of PTCH1 drug efflux activity enhances chemotherapy efficacy against triple negative breast cancers

doi: 10.1016/j.tranon.2026.102777

Figure Lengend Snippet: PAH increases docetaxel efficacy against TNBC spheroids. MDA-MB-231 cells were plated in ultra-low attachment surface 24 well plates in complete medium and treated with increasing concentrations of docetaxel in the presence of DMSO (control, 0 PAH), PAH 10 µM or 30 µM. After 2 weeks pictures were taken using Cytation 5 cell imaging system from Biotek. The surface of spheroids was calculated and reported after normalization on the condition without docetaxel for each concentration of PAH.

Article Snippet: Human breast cancer cell lines MDA-MB-231, MDA-MB-468 and HCC-38 were purchased from ATCC.

Techniques: Control, Imaging, Concentration Assay

Journal: Translational Oncology

Article Title: Inhibition of PTCH1 drug efflux activity enhances chemotherapy efficacy against triple negative breast cancers

doi: 10.1016/j.tranon.2026.102777

Figure Lengend Snippet: PAH addition to chemotherapy inhibits migration of TNBC cells. A. 100000 cells were seeded on membrane from Transwell plates. Cells were treated 24 h with doxorubicin ± 15µM PAH or 48 h with docetaxel ± 15µM PAH. After fixation and staining with crystal violet, wells were observed on a microscope with X5 objective. B. Migration was measured using a wound-healing assay. A wound was performed on MDA-MB-231 confluent cells seeded in 24 well plates. The medium was replaced by fresh medium containing 5 µM docetaxel in the absence of PAH, or the presence of 5 µM PAH. Two pictures were taken at two different points of each well immediately after wound, and 3 days after wound with 5X objective. The width of the wound was measured using ImageJ software and reported as final wound width/initial wound width in percentage. Data presented are the mean ± SEM of 3 independent experiments. Significance is attained at P < 0.05.

Article Snippet: Human breast cancer cell lines MDA-MB-231, MDA-MB-468 and HCC-38 were purchased from ATCC.

Techniques: Migration, Membrane, Staining, Microscopy, Wound Healing Assay, Software

Journal: Translational Oncology

Article Title: Inhibition of PTCH1 drug efflux activity enhances chemotherapy efficacy against triple negative breast cancers

doi: 10.1016/j.tranon.2026.102777

Figure Lengend Snippet: PTCH1 contributes to the efflux of doxorubicin from TNBC cells. A. PTCH1 protein expression (left panel) and intracellular doxorubicin (right panel) were analyzed 16 h after transfection of MDA-MB-231 cells with PTCH1-siRNA or negative-control-siRNA. PTCH1 and GAPDH western blot signals were quantified using ImageJ software (left panel). For doxorubicin accumulation measurements (right panel), MDA-MB-231 cells were grown on slides and transfected with PTCH1-siRNA or negative-control-siRNA. After incubation with 10 µM doxorubicin, 3 coverslips were fixed for doxorubicin loading control; the other coverslips were incubated with efflux buffer and fixed. B. Docetaxel inhibits the accumulation of doxorubicin in MDA-MB-231 cells. Cells on coverslip were incubated with 10 µM doxorubicin or 10 µM doxorubicin and 50 µM docetaxel. Doxorubicin fluorescence images were acquired by epifluorescence microscopy using a 40X objective, and doxorubicin fluorescence was quantified using ImageJ software for about 100 cells per condition per experiment. Histograms are the mean ± SEM of 3 independent experiments. Significance is attained at P < 0.05.

Article Snippet: Human breast cancer cell lines MDA-MB-231, MDA-MB-468 and HCC-38 were purchased from ATCC.

Techniques: Expressing, Transfection, Negative Control, Western Blot, Software, Incubation, Control, Fluorescence, Epifluorescence Microscopy

Journal: ACS Pharmacology & Translational Science

Article Title: Engineering the Host Defense Peptide from Scorpion Venom for Safer and More Potent Antibreast Cancer Activity

doi: 10.1021/acsptsci.5c00598

Figure Lengend Snippet: Cell viability of the cell lines MCF-10A (A), MCF-7 (B), and MDA-MB-231 (C) after treatments with IsCT1 (green bars) or AKFK-IsCT1 (purple bars) at concentrations up to 4.0 μM, followed by incubation at 37 °C and 5% CO 2 for 4 h. Green bars represent IsCT1, and pink bars represent AKFK-IsCT1. Data are expressed as mean ± SD of three independent experiments performed in triplicate. Statistical significance was determined using two-way ANOVA followed by Tukey test, ** p < 0.005; **** p < 0.0001.

Article Snippet: The triple-negative breast cancer (TNBC) cell lines human MDA-MB-231 (CRM-HTB-26) and murine 4T1 (CRL-2539), human adenocarcinoma cell line MCF-7 (HTB-22), and the human breast epithelial cell line MCF-10A (CRL-10317) were obtained from American Type Culture Collection (ATCC).

Techniques: Incubation

Journal: ACS Pharmacology & Translational Science

Article Title: Engineering the Host Defense Peptide from Scorpion Venom for Safer and More Potent Antibreast Cancer Activity

doi: 10.1021/acsptsci.5c00598

Figure Lengend Snippet: Cell viability after combined treatment with photodynamic therapy using hypericin (PDT-Hyp, 0.05 μM) and peptides under two treatment protocols. Protocol 1 (PDT-Hyp followed by peptide exposure) was applied to (A) MCF-10A, (B) MCF-7, and (C) MDA-MB-231 cells, whereas Protocol 2 (peptide exposure followed by PDT-Hyp) was applied to (D) MCF-10A, (E) MCF-7, and (F) MDA-MB-231 cells. Blue bars represent IsCT1, and red bars represent AKFK-IsCT1. Peptides were tested at concentrations ranging from 0.01 to 4.0 μM. Cells were incubated at 37 °C and 5% CO 2 for 4 h following treatment. Data are expressed as mean ± SD of three independent experiments performed in triplicate. Statistical significance was determined using two-way ANOVA followed by Tukey test, * p < 0.05; ** p < 0.005; *** p < 0.0005; **** p < 0.0001.

Article Snippet: The triple-negative breast cancer (TNBC) cell lines human MDA-MB-231 (CRM-HTB-26) and murine 4T1 (CRL-2539), human adenocarcinoma cell line MCF-7 (HTB-22), and the human breast epithelial cell line MCF-10A (CRL-10317) were obtained from American Type Culture Collection (ATCC).

Techniques: Incubation

Journal: ACS Pharmacology & Translational Science

Article Title: Engineering the Host Defense Peptide from Scorpion Venom for Safer and More Potent Antibreast Cancer Activity

doi: 10.1021/acsptsci.5c00598

Figure Lengend Snippet: Three-dimensional Bliss synergy response surfaces for the combined treatment of PDT-Hyp and peptides applied under different treatment protocols. Protocol 1 was evaluated with IsCT1 (A, E, I) or AKFK-IsCT1 (C, G, K), whereas Protocol 2 was evaluated with IsCT1 (B, F, J) or AKFK-IsCT1 (D, H, L). Panels in the first row (A–D) correspond to MCF-10A cells, the second row (E–H) to MCF-7 cells, and the third row (I–L) to MDA-MB-231 cells. Color coding represents Bliss synergy scores, where green indicates antagonism (≤0), intermediate colors between white and light red indicate additive effects (>0 and ≤10), and red indicates synergistic interactions (>10). Color intensity is proportional to the magnitude of the interaction score. Bliss synergy scores and response surfaces were generated from complete dose–response matrices using SynergyFinder 3.0.

Article Snippet: The triple-negative breast cancer (TNBC) cell lines human MDA-MB-231 (CRM-HTB-26) and murine 4T1 (CRL-2539), human adenocarcinoma cell line MCF-7 (HTB-22), and the human breast epithelial cell line MCF-10A (CRL-10317) were obtained from American Type Culture Collection (ATCC).

Techniques: Generated